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Provedor de dados:  BJM
País:  Brazil
Título:  Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes
Autores:  Luz,José Maria Rodrigues da
Nunes,Mateus Dias
Paes,Sirlaine Albino
Torres,Denise Pereira
Silva,Marliane de Cássia Soares da
Kasuya,Maria Catarina Megumi
Data:  2012-12-01
Ano:  2012
Palavras-chave:  Laccase
Cellulase
Mushrooms
Lignocellulolytic residues
Resumo:  The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the lignocellulolytic enzymes produced by two P. ostreatus strains (PLO 2 and PLO 6). These strains were used to inoculate samples of coffee husks, eucalyptus sawdust or eucalyptus bark add with or without 20 % rice bran. Every five days after substrate inoculation, the enzyme activity and soluble protein concentration were evaluated. The maximum activity of oxidative enzymes was observed at day 10 after inoculation, and the activity of the hydrolytic enzymes increased during the entire period of the experiment. The results show that substrate composition and colonization time influenced the activity of the lignocellulolytic enzymes.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822012000400035
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.43 n.4 2012
Direitos:  info:eu-repo/semantics/openAccess
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